مقایسه تأثیر هشت هفته تمرین هوازی و مقاومتی به همراه مصرف مکمل کوئرستین بر سطح فاکتور رشد اندوتلیال عروقی (VEGF) بافت قلب در رت‌های نر نژاد ویستار

نوع مقاله : مقاله علمی - پژوهشی

نویسندگان

1 استاد دانشگاه تربیت دبیر شهید رجایی، تهران، ایران

2 دانشجوی کارشناسی ارشد فیزیولوژی تغذیه، دانشگاه تربیت دبیر شهید رجایی، تهران، ایران

3 دانشیار دانشگاه تربیت دبیر شهید رجایی، تهران، ایران

چکیده

مقدمه: فعالیت ورزشی از طریق کاهش عوامل خطرزای سلامتی، نقش مؤثری در سلامت جسمی و روانی دارد. هدف از پژوهش حاضر، مقایسه تأثیر هشت هفته تمرین هوازی و مقاومتی به همراه مکمل کوئرستین بر VEGF بافت قلب در رت­ها بود.
مواد و روش­ها: 35 سر رت 10 – 8 هفته­ای به صورت تصادفی به 7 گروه هوازی، مقاومتی، هوازی + مکمل، مقاومتی + مکمل، مکمل، شم و کنترل تقسیم شدند. پس از دو هفته آشناسازی با محیط و یادگیری تمرین گروه­های تمرینی برای 8 هفته طبق پروتکل به تمرین پرداختند. گروه­هایی که باید مکمل دریافت می­کردند، مکمل کوئرستین را به میزان 50 میلی­گرم بر کیلوگرم، سه روز در هفته و به مدت 8 هفته بصورت تزریق صفاقی دریافت کردند. 48 ساعت پس از آخرین مداخله تمرین، با استفاده از گاز CO2 بیهوش و کشته شدند. سپس نمونه­گیری از قلب آنها انجام شد. برای اندازه­گیری غلظت VEGF از روش الایزا استفاده شد. برای تجزیه و تحلیل داده­ها از آزمون تحلیل واریانس یک طرفه و آزمون تعقیبی توکی در سطح 05/0 ≥ P با استفاده از SPSS 22 استفاده شد.
یافته ­ها: یافته­های تحقیق نشان داد که هشت هفته تمرین هوازی و مقاومتی و هوازی به همراه مکمل کوئرستین، باعث افزایش معنی­دار غلظت VEGF قلب رت­ها، نسبت به گروه کنترل می­شود (05/0 ≥ P )، ولی در گروه مکمل سطح VEGF در بافت قلب نسبت به گروه کنترل تفاوت معنی­داری نداشت (148/0 = P).
نتیجه­ گیری: به طور کلی، مصرف کوئرستین سبب مهار آنژیوژنز می­شود ولی در گروه مکمل +  هوازی این اتفاق رخ نداد (011/0 = P) یعنی فعالیت هوازی بر اثرات مهاری کوئرستین بر آنژیوژنز غلبه کرده است.

کلیدواژه‌ها


عنوان مقاله [English]

Comparison of the Effect of Eight Weeks of Aerobic and Resistance Training with Quercetin Supplementation on Vascular Endothelial Growth Factor (VEGF) in Cardiac Tissue of Male Wistar Rats

نویسندگان [English]

  • M. Kashef 1
  • F. Bahadori 2
  • F. Shahidi 3
1 Professor of Shahid Rajaee Teacher Training University, Tehran, Iran.
2 MSc Student of Nutritional Physiology, Shahid Rajaee Teacher Training University, Tehran, Iran.
3 Associate Professor of Shahid Rajaee Teacher Training University, Tehran, Iran.
چکیده [English]

Introduction: Exercise has an effective role in physical and mental health by reducing the health risk factors.
Materials and Methods: The study aimed to compare the effect of eight weeks of aerobic and resistance training with quercetin supplementation on VEGF of cardiac tissue in rats. 35 Rats with an age average of 8-10 weeks were randomly divided into 7 groups (aerobic, resistance, aerobic + supplement, resistance + supplement, supplement, sham, and control). After two weeks of familiarity with the environment and learning the exercise, The Exercise groups practiced according to the protocol for 8 weeks. The groups that should have received supplements received quercetin supplement at the rate of 50 mg/kg three days a week for 8 weeks as a peritoneal injection. 48 hours after the last training intervention, rats were anesthetized and killed using CO2. The, samples were taken from the heart of rats. ELISA method was used to measure VEGF concentration. One-way ANOVA and Tukey Post Hoc test in P≤0.05 level by SPSS 22 was employed.
Results: The results showed that eight weeks of aerobic and resistance training and aerobic with quercetin significantly increased the VEGF concentration of rat heart compared to the control group (P≤0.05), while, there was no significant difference in the level of VEGF in heart tissue compared to the control group and supplement (P=0.148).
Conclusion: In general, quercetin consumption inhibited angiogenesis, while, it did not happen in the supplement + aerobic group (P=0.011), that means that aerobic has overcome the inhibitory effects of quercetin on angiogenesis.
 

کلیدواژه‌ها [English]

  • Exercise
  • Quercetin
  • Rat
  • Vascular Endothelial Growth Factor (VEGF)
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